منابع مشابه
The Movement of Membranous Organelles in Axons
To identify the structures to be rapidly transported through the axons, we developed a new method to permit local cooling of mouse saphenous nerves in situ without exposing them . By this method, both anterograde and retrograde transport were successfully interrupted, while the structural integrity of the nerves was well preserved . Using radioactive tracers, anterogradely transported proteins ...
متن کاملKinesin associates with anterogradely transported membranous organelles in vivo
Biochemical, pharmacological and immunocytochemical studies have implicated the microtubule-activated ATPase, kinesin, in the movement of membrane bounded organelles in fast axonal transport. In vitro studies suggested that kinesin moves organelles preferentially in the anterograde direction, but data about the function and precise localization of kinesin in the living axon were lacking. The cu...
متن کاملThe movement of membranous organelles in axons. Electron microscopic identification of anterogradely and retrogradely transported organelles
To identify the structures to be rapidly transported through the axons, we developed a new method to permit local cooling of mouse saphenous nerves in situ without exposing them. By this method, both anterograde and retrograde transport were successfully interrupted, while the structural integrity of the nerves was well preserved. Using radioactive tracers, anterogradely transported proteins we...
متن کاملAn Intracellular Nanotrap Redirects Proteins and Organelles in Live Bacteria
UNLABELLED Owing to their small size and enhanced stability, nanobodies derived from camelids have previously been used for the construction of intracellular "nanotraps," which enable redirection and manipulation of green fluorescent protein (GFP)-tagged targets within living plant and animal cells. By taking advantage of intracellular compartmentalization in the magnetic bacterium Magnetospiri...
متن کاملChanges in the distribution of membranous organelles during mouse early development.
The unfertilized oocyte, fertilized egg and early embryo (2-cell to 16-cell) of the mouse have been examined immunocytochemically for the distribution of antigens associated with the endoplasmic reticulum, the lysosomal and acidic vesicle fraction (100 kD antigen), Golgi apparatus (135 kD antigen) and coated vesicles (clathrin). The distribution of these antigens has also been examined in isola...
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ژورنال
عنوان ژورنال: Journal of Molecular Microbiology and Biotechnology
سال: 2013
ISSN: 1660-2412,1464-1801
DOI: 10.1159/000346496